Mutations affecting retinotectal axonal pathfinding in Medaka, Oryzias latipes

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Mutations affecting retinotectal axonal pathfinding in Medaka, Oryzias latipes

We screened for mutations affecting retinotectal axonal projection in Medaka, Oryzias latipes. In wild-type Medaka embryos, all the axons of retinal ganglion cells (RGCs) project to the contralateral tectum, such that the topological relationship of the retinal field is maintained. We labeled RGC axons using DiI/DiO at the nasodorsal and temporoventral positions of the retina, and screened for ...

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The metameric structure of the vertebrate trunk is generated by repeated formation of somites from the unsegmented presomitic mesoderm (PSM). We report the initial characterization of nine different mutants affecting segmentation that were isolated in a large-scale mutagenesis screen in Medaka (Oryzias latipes). Four mutants were identified that show a complete or partial absence of somites or ...

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Zebrafish mutations affecting retinotectal axon pathfinding.

We have isolated mutants in the zebrafish Danio rerio that have defects in axonal connectivity between the retina and tectum. 5-day-old fish larvae were screened by labeling retinal ganglion cells with DiI and DiO and observing their axonal projections to and on the tectum. 82 mutations, representing 13 complementation groups and 6 single allele loci, were found that have defects in retinal gan...

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ژورنال

عنوان ژورنال: Mechanisms of Development

سال: 2004

ISSN: 0925-4773

DOI: 10.1016/j.mod.2004.03.021